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Unintended perturbation of protein function using GFP nanobodies in human cells

Tagging a protein of interest with GFP using genome editing is a popular approach to study protein function in cell and developmental biology. To avoid re-engineering cell lines or organisms in order to introduce additional tags, functionalized nanobodies that bind GFP can be used to extend the func...

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Detalhes bibliográficos
Publicado no:J Cell Sci
Main Authors: Küey, Cansu, Larocque, Gabrielle, Clarke, Nicholas I., Royle, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists Ltd 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6857592/
https://ncbi.nlm.nih.gov/pubmed/31601614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.234955
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