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Mitochondrial respiration and H(2)O(2) emission in saponin-permeabilized murine diaphragm fibers: optimization of fiber separation and comparison to limb muscle

Diaphragm abnormalities in aging or chronic diseases include impaired mitochondrial respiration and H(2)O(2) emission, which can be measured using saponin-permeabilized muscle fibers. Mouse diaphragm presents a challenge for isolation of fibers due to relatively high abundance of connective tissue i...

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Detalhes bibliográficos
Publicado no:Am J Physiol Cell Physiol
Main Authors: Hahn, Dongwoo, Kumar, Ravi A., Ryan, Terence E., Ferreira, Leonardo F.
Formato: Artigo
Idioma:Inglês
Publicado em: American Physiological Society 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6851001/
https://ncbi.nlm.nih.gov/pubmed/31314583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpcell.00184.2019
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