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Resistance of Dictyostelium discoideum membranes to saponin permeabilization

BACKGROUND: Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae. FINDINGS: We show that saponin, contrary to Triton X-100 or alcohol, permeabiliz...

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Bibliografiska uppgifter
Huvudupphovsmän: Mercanti, Valentina, Cosson, Pierre
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2010
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2877058/
https://ncbi.nlm.nih.gov/pubmed/20426861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-120
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