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Resistance of Dictyostelium discoideum membranes to saponin permeabilization
BACKGROUND: Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae. FINDINGS: We show that saponin, contrary to Triton X-100 or alcohol, permeabiliz...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2877058/ https://ncbi.nlm.nih.gov/pubmed/20426861 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-120 |
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