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Homologous Recombination-Based Genome Editing by Clade F AAVs Is Inefficient in the Absence of a Targeted DNA Break

Adeno-associated virus (AAV) vectors are frequently used as donor templates for genome editing by homologous recombination. Although modification rates are typically under 1%, they are greatly enhanced by targeted double-stranded DNA breaks (DSBs). A recent report described clade F AAVs mediating hi...

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Vydáno v:Mol Ther
Hlavní autoři: Rogers, Geoffrey L., Chen, Hsu-Yu, Morales, Heidy, Cannon, Paula M.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society of Gene & Cell Therapy 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6822228/
https://ncbi.nlm.nih.gov/pubmed/31540849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2019.08.019
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