Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

BACKGROUND: Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious. Such a method is still used to measure potency of live Oral Poliovirus Vaccine du...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Virol J
Main Authors: Manukyan, Hasmik, Rodionova, Elvira, Zagorodnyaya, Tatiana, Lin, Tsai-Lien, Chumakov, Konstantin, Laassri, Majid
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2019
Teme:
Methodology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6819588/
https://ncbi.nlm.nih.gov/pubmed/31660997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12985-019-1233-6
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