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The effect of reverse transcription enzymes and conditions on high throughput amplicon sequencing of the 16S rRNA

It is assumed that the sequencing of ribosomes better reflects the active microbial community than the sequencing of the ribosomal RNA encoding genes. Yet, many studies exploring microbial communities in various environments, ranging from the human gut to deep oceans, questioned the validity of this...

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Bibliografiske detaljer
Udgivet i:PeerJ
Main Authors: Šťovíček, Adam, Cohen-Chalamish, Smadar, Gillor, Osnat
Format: Artigo
Sprog:Inglês
Udgivet: PeerJ Inc. 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6816399/
https://ncbi.nlm.nih.gov/pubmed/31667010
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.7608
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