Spectroscopic characterization of the EF-hand domain of phospholipase C δ1: Identification of a lipid interacting domain

The interaction of the isolated EF-hand domain of phospholipase C δ1 with arachidonic acid (AA) was characterized using circular dichroism (CD) and fluorescence spectroscopy. The far-UV CD spectral changes indicate that AA binds to the EF domain. The near-UV CD spectra suggest that the orientations...

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Publicado no:Arch Biochem Biophys
Main Authors: Kobayashi, Minae, Gryczynski, Zygmunt, Lukomska, Joanna, Feng, Jianwen, Roberts, Mary F., Lakowicz, Joseph R., Lomasney, Jon W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2005
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6814010/
https://ncbi.nlm.nih.gov/pubmed/16054586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2005.06.014
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