piggyBac Transposon-Based Immortalization of Human Deciduous Tooth Dental Pulp Cells with Multipotency and Non-Tumorigenic Potential

We aimed to immortalize primarily isolated human deciduous tooth-derived dental pulp cells (HDDPCs) by transfection with piggyBac (PB)-based transposon vectors carrying E7 from human papilloma virus 16 or complementary DNA (cDNA) encoding human telomerase reverse transcriptase (hTERT). HDDPCs were c...

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Veröffentlicht in:Int J Mol Sci
Hauptverfasser: Inada, Emi, Saitoh, Issei, Kubota, Naoko, Iwase, Yoko, Kiyokawa, Yuki, Shibasaki, Shinji, Noguchi, Hirofumi, Yamasaki, Youichi, Sato, Masahiro
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6801629/
https://ncbi.nlm.nih.gov/pubmed/31623314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20194904
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