Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae

OBJECTIVE: The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl(2)), applicable for immunofluorescence assay. RESULTS: Herein we optim...

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Bibliografiske detaljer
Udgivet i:BMC Res Notes
Main Authors: Danchenko, Monika, Csaderova, Lucia, Fournier, Pierre Edouard, Sekeyova, Zuzana
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Research Note
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6794859/
https://ncbi.nlm.nih.gov/pubmed/31619275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-019-4699-9
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