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Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae
OBJECTIVE: The objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES, HEPES, EGTA and MgCl(2)), applicable for immunofluorescence assay. RESULTS: Herein we optim...
Salvato in:
| Pubblicato in: | BMC Res Notes |
|---|---|
| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6794859/ https://ncbi.nlm.nih.gov/pubmed/31619275 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-019-4699-9 |
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