Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry

EPR spectroscopy of diamagnetic bio‐macromolecules is based on site‐directed spin labeling (SDSL). Herein, a novel labeling strategy for proteins is presented. A nitroxide‐based spin label has been developed and synthesized that can be ligated to proteins by an inverse‐electron‐demand Diels–Alder (D...

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Publicat a:Chembiochem
Autors principals: Kugele, Anandi, Silkenath, Bjarne, Langer, Jakob, Wittmann, Valentin, Drescher, Malte
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2019
Matèries:
Communications
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6790680/
https://ncbi.nlm.nih.gov/pubmed/31090999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900318
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