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Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enableing simultaneous quantification of the lesions on individual DNA molecules....

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Publicado en:Chem Commun (Camb)
Autores principales: Torchinsky, Dmitry, Michaeli, Yael, Gassman, Natalie R., Ebenstein, Yuval
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6783632/
https://ncbi.nlm.nih.gov/pubmed/31482872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9cc05198h
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