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Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enableing simultaneous quantification of the lesions on individual DNA molecules....

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Publicat a:Chem Commun (Camb)
Autors principals: Torchinsky, Dmitry, Michaeli, Yael, Gassman, Natalie R., Ebenstein, Yuval
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6783632/
https://ncbi.nlm.nih.gov/pubmed/31482872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9cc05198h
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