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Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enableing simultaneous quantification of the lesions on individual DNA molecules....

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Détails bibliographiques
Publié dans:Chem Commun (Camb)
Auteurs principaux: Torchinsky, Dmitry, Michaeli, Yael, Gassman, Natalie R., Ebenstein, Yuval
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6783632/
https://ncbi.nlm.nih.gov/pubmed/31482872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9cc05198h
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