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Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enableing simultaneous quantification of the lesions on individual DNA molecules....

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Bibliographic Details
Published in:Chem Commun (Camb)
Main Authors: Torchinsky, Dmitry, Michaeli, Yael, Gassman, Natalie R., Ebenstein, Yuval
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6783632/
https://ncbi.nlm.nih.gov/pubmed/31482872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9cc05198h
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