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Probing in vivo structure of individual mRNA 3’ isoforms using dimethyl sulfate

The DREADS (DMS Region Extraction and Deep Sequencing) procedure was designed to probe RNA structure in vivo and to link this structural information to specific 3’ isoforms. Growing cells are treated with the alkylating agent dimethyl sulfate (DMS), which enters easily into cells and modifies RNA mo...

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Detalles Bibliográficos
Publicado en:Curr Protoc Mol Biol
Main Authors: Moqtaderi, Zarmik, Geisberg, Joseph V.
Formato: Artigo
Idioma:Inglês
Publicado: 2019
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6777956/
https://ncbi.nlm.nih.gov/pubmed/31503415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.99
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