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Probing in vivo structure of individual mRNA 3’ isoforms using dimethyl sulfate
The DREADS (DMS Region Extraction and Deep Sequencing) procedure was designed to probe RNA structure in vivo and to link this structural information to specific 3’ isoforms. Growing cells are treated with the alkylating agent dimethyl sulfate (DMS), which enters easily into cells and modifies RNA mo...
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| Publicat a: | Curr Protoc Mol Biol |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6777956/ https://ncbi.nlm.nih.gov/pubmed/31503415 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.99 |
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