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Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

Targeted gene knockouts play an important role in the study of gene function. For the generation of knockouts in the industrially important yeast Pichia pastoris, several protocols have been published to date. Nevertheless, creating a targeted knockout in P. pastoris still is a time‐consuming proces...

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Dettagli Bibliografici
Pubblicato in:Yeast
Autori principali: Ahmad, Mudassar, Winkler, Christine M., Kolmbauer, Markus, Pichler, Harald, Schwab, Helmut, Emmerstorfer‐Augustin, Anita
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2019
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6771850/
https://ncbi.nlm.nih.gov/pubmed/31148217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3426
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