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Direct wavefront sensing enables functional imaging of infragranular axons and spines

We advance two-photon microscopy for near diffraction-limited imaging down to 850 μm below the pia in awake mice. Our approach combines direct wavefront sensing of descanned fluorescence from Cy5.5-dextran in brain microvessels, which forms a guide star, and adaptive optics to compensate for tissue-...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Liu, Rui, Li, Zengyi, Marvin, Jonathan S., Kleinfeld, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6763395/
https://ncbi.nlm.nih.gov/pubmed/31209383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0434-7
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