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Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1
Stable cell lines can continuously produce a recombinant protein without the need to repeatedly engineer the genome. In a previous study HIPK1, Homeodomain-interacting Protein Kinase 1, was found to be a target of the microRNA miR-22 that, when repressed, improved expression of both an intracellular...
Kaydedildi:
| Yayımlandı: | N Biotechnol |
|---|---|
| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2019
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6759407/ https://ncbi.nlm.nih.gov/pubmed/31425885 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.nbt.2019.08.004 |
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