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In Situ Single-Cell Western Blot on Adherent Cell Culture

Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate trypsin release of cells to suspension, prior to single-cell isolation and protein analysis. To assay HeLa cells from an attached starting state, we culture adher...

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Dades bibliogràfiques
Publicat a:Angew Chem Int Ed Engl
Autors principals: Zhang, Yizhe, Naguro, Isao, Herr, Amy E.
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6759404/
https://ncbi.nlm.nih.gov/pubmed/31390130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201906920
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