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In Situ Single-Cell Western Blot on Adherent Cell Culture
Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate trypsin release of cells to suspension, prior to single-cell isolation and protein analysis. To assay HeLa cells from an attached starting state, we culture adher...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6759404/ https://ncbi.nlm.nih.gov/pubmed/31390130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201906920 |
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