Integration of a FT expression cassette into CRISPR/Cas9 construct enables fast generation and easy identification of transgene-free mutants in Arabidopsis

The CRISPR/Cas9 genome editing technique has been widely used to generate transgene-free mutants in different plant species. Several different methods including fluorescence marker-assisted visual screen of transgene-free mutants and programmed self-elimination of CRISPR/Cas9 construct have been use...

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Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: Cheng, Yuxin, Zhang, Na, Hussain, Saddam, Ahmed, Sajjad, Yang, Wenting, Wang, Shucai
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2019
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6756540/
https://ncbi.nlm.nih.gov/pubmed/31545795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0218583
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