Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets

A new approach to single-molecule DNA sequencing in which dNTPs, released by pyrophosphorolysis from the strand to be sequenced, are captured in microdroplets and read directly could have substantial advantages over current sequence-by-synthesis methods; however, there is no existing method sensitiv...

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Pubblicato in:Nucleic Acids Res
Autori principali: Breiner, Boris, Johnson, Kerr, Stolarek, Magdalena, Silva, Ana-Luisa, Negrea, Aurel, Bell, Neil M, Isaac, Tom H, Dethlefsen, Mark, Chana, Jasmin, Ibbotson, Lindsey A, Palmer, Rebecca N, Bush, James, Dunning, Alexander J, Love, David M, Pachoumi, Olympia, Kelly, Douglas J, Shibahara, Aya, Wu, Mei, Sosna, Maciej, Dear, Paul H, Tolle, Fabian, Petrini, Edoardo, Amasio, Michele, Shelford, Leigh R, Saavedra, Monica S, Sheridan, Eoin, Kuleshova, Jekaterina, Podd, Gareth J, Balmforth, Barnaby W, Frayling, Cameron A
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2019
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6753480/
https://ncbi.nlm.nih.gov/pubmed/31318971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz611
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