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Florescence in situ imaging of dendritic RNAs at single molecule resolution

RNA localization is an important step in gene regulation. Imaging RNAs in fixed and live cells provides contextual information about RNA distribution in the cells. Here we provide a detailed protocol for performing a single molecule florescent in situ hybridization technique (smFISH). smFISH detects...

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Bibliografiske detaljer
Udgivet i:Curr Protoc Neurosci
Main Authors: Batish, Mona, Tyagi, Sanjay
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6752217/
https://ncbi.nlm.nih.gov/pubmed/31532916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpns.79
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