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Comparative evaluation of RNA-Seq library preparation methods for strand-specificity and low input

Library preparation is a key step in sequencing. For RNA sequencing there are advantages to both strand specificity and working with minute starting material, yet until recently there was no kit available enabling both. The Illumina TruSeq stranded mRNA Sample Preparation kit (TruSeq) requires abund...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Sarantopoulou, Dimitra, Tang, Soon Yew, Ricciotti, Emanuela, Lahens, Nicholas F., Lekkas, Damien, Schug, Jonathan, Guo, Xiaofeng S., Paschos, Georgios K., FitzGerald, Garret A., Pack, Allan I., Grant, Gregory R.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6748930/
https://ncbi.nlm.nih.gov/pubmed/31530843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49889-1
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