Comparative evaluation of RNA-Seq library preparation methods for strand-specificity and low input

Library preparation is a key step in sequencing. For RNA sequencing there are advantages to both strand specificity and working with minute starting material, yet until recently there was no kit available enabling both. The Illumina TruSeq stranded mRNA Sample Preparation kit (TruSeq) requires abund...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Sarantopoulou, Dimitra, Tang, Soon Yew, Ricciotti, Emanuela, Lahens, Nicholas F., Lekkas, Damien, Schug, Jonathan, Guo, Xiaofeng S., Paschos, Georgios K., FitzGerald, Garret A., Pack, Allan I., Grant, Gregory R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6748930/
https://ncbi.nlm.nih.gov/pubmed/31530843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49889-1
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