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Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

BACKGROUND: Antibody fragments can be expressed in Escherichia coli, where they are commonly directed to the periplasm via Sec pathway to enable disulphide bridge formations and correct folding. In order to transport antibody fragments to the periplasmic space via Sec pathway, they are equipped with...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Microb Cell Fact
Prif Awduron: Kulmala, Antti, Huovinen, Tuomas, Lamminmäki, Urpo
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2019
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6745802/
https://ncbi.nlm.nih.gov/pubmed/31526395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1210-1
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