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Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries
BACKGROUND: Antibody fragments can be expressed in Escherichia coli, where they are commonly directed to the periplasm via Sec pathway to enable disulphide bridge formations and correct folding. In order to transport antibody fragments to the periplasmic space via Sec pathway, they are equipped with...
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| Pubblicato in: | Microb Cell Fact |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6745802/ https://ncbi.nlm.nih.gov/pubmed/31526395 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1210-1 |
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