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Gata4基因H435Y突变型小鼠模型的建立及验证

OBJECTIVE: To establish a mouse model of H435Y mutation of Gata4 gene using CRISPR/Cas9- mediated gene targeting. METHODS: The single-stranded guide RNA (sgRNA) specific to the H435Y loci of Gata4 gene was designed based on the sequence of Gata4 gene. After activity assessment, the active sgRNA and...

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Bibliografiska uppgifter
I publikationen:Nan Fang Yi Ke Da Xue Xue Bao
Materialtyp: Artigo
Språk:Inglês
Publicerad: 南方医科大学学报编辑部 2018
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6744054/
https://ncbi.nlm.nih.gov/pubmed/30377126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3969/j.issn.1673-4254.2018.10.15
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