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Biological surface properties in extracellular vesicles and their effect on cargo proteins
Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/ https://ncbi.nlm.nih.gov/pubmed/31506490 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3 |
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