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Biological surface properties in extracellular vesicles and their effect on cargo proteins

Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Santucci, Laura, Bruschi, Maurizio, Del Zotto, Genny, Antonini, Francesca, Ghiggeri, Gian Marco, Panfoli, Isabella, Candiano, Giovanni
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/
https://ncbi.nlm.nih.gov/pubmed/31506490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3
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