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Biological surface properties in extracellular vesicles and their effect on cargo proteins
Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...
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| Veröffentlicht in: | Sci Rep |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group UK
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/ https://ncbi.nlm.nih.gov/pubmed/31506490 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3 |
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