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Biological surface properties in extracellular vesicles and their effect on cargo proteins

Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Santucci, Laura, Bruschi, Maurizio, Del Zotto, Genny, Antonini, Francesca, Ghiggeri, Gian Marco, Panfoli, Isabella, Candiano, Giovanni
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/
https://ncbi.nlm.nih.gov/pubmed/31506490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3
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