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Biological surface properties in extracellular vesicles and their effect on cargo proteins
Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...
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| Publicado en: | Sci Rep |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/ https://ncbi.nlm.nih.gov/pubmed/31506490 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3 |
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