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Biological surface properties in extracellular vesicles and their effect on cargo proteins

Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Santucci, Laura, Bruschi, Maurizio, Del Zotto, Genny, Antonini, Francesca, Ghiggeri, Gian Marco, Panfoli, Isabella, Candiano, Giovanni
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6736982/
https://ncbi.nlm.nih.gov/pubmed/31506490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-47598-3
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