Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging

Whole-genome bisulfite sequencing (WGBS) is the current gold standard of methylome analysis. Post-bisulfite adaptor tagging (PBAT) is an increasingly popular WGBS protocol because of high sensitivity and low bias. PBAT originally relied on two rounds of random priming for adaptor-tagging of single-s...

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Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Miura, Fumihito, Shibata, Yukiko, Miura, Miki, Sangatsuda, Yuhei, Hisano, Osamu, Araki, Hiromitsu, Ito, Takashi
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2019
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6736019/
https://ncbi.nlm.nih.gov/pubmed/31114914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz435
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