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Crisflash: open-source software to generate CRISPR guide RNAs against genomes annotated with individual variation
SUMMARY: CRISPR/Cas9 system requires short guide RNAs (sgRNAs) to direct genome modification. Most currently available tools for sgRNA design operate only with standard reference genomes, and are best suited for small-scale projects. To address these limitations, we developed Crisflash, a software t...
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| Veröffentlicht in: | Bioinformatics |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6735888/ https://ncbi.nlm.nih.gov/pubmed/30649181 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bioinformatics/btz019 |
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