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An Improved Two-Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells

We previously established a two-step protocol for differentiation of human pluripotent stem cells (hPSC) into trophoblast, using a StemPro-based minimal media (EMIM) with Bone Morphogenetic Protein-4 (BMP4). However, this protocol was suboptimal, resulting in induction of mixed mesoderm and trophobl...

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Bibliographische Detailangaben
Veröffentlicht in:Curr Protoc Stem Cell Biol
Hauptverfasser: Horii, Mariko, Bui, Tony, Touma, Ojeni, Cho, Hee Young, Parast, Mana M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6730560/
https://ncbi.nlm.nih.gov/pubmed/31479595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.96
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