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A glycal-based photoaffinity probe that enriches sialic acid binding proteins

To identify sialic acid binding proteins from complex proteomes, three photocrosslinking affinity-based probes were constructed using Neu5Ac (5 and 6) and Neu5Ac2en (7) scaffolds. Kinetic inhibition assays and Western blotting revealed the Neu5Ac2en-based 7 to be an effective probe for the labeling...

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Detalhes bibliográficos
Publicado no:Bioorg Med Chem Lett
Main Authors: Thuy-Boun, Peter S., Wolan, Dennis W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6730559/
https://ncbi.nlm.nih.gov/pubmed/31387789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2019.07.054
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