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Optimization of Enzymatic Antibody Fragmentation for Yield, Efficiency, and Binding Affinity

Enzymatic antibody fragmentation has been well studied for various hosts and isotypes, but fragmentation patterns also vary unpredictably by clone, and optimizing Fab or F(ab’)(2) production by trial and error consumes large quantities of antibodies. Here, we report a systematic strategy for optimiz...

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Bibliografiske detaljer
Udgivet i:Bioconjug Chem
Main Authors: Kinman, Andrew W.L., Pompano, Rebecca R.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6718091/
https://ncbi.nlm.nih.gov/pubmed/30649877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.bioconjchem.8b00912
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