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Optimization of Enzymatic Antibody Fragmentation for Yield, Efficiency, and Binding Affinity
Enzymatic antibody fragmentation has been well studied for various hosts and isotypes, but fragmentation patterns also vary unpredictably by clone, and optimizing Fab or F(ab’)(2) production by trial and error consumes large quantities of antibodies. Here, we report a systematic strategy for optimiz...
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| Publicat a: | Bioconjug Chem |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6718091/ https://ncbi.nlm.nih.gov/pubmed/30649877 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.bioconjchem.8b00912 |
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