A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology

Knock-in (KI) gene targeting can be employed for a wide range of applications in stem cell research. However, vectors for KI require multiple complicated processes for construction, including multiple times of digestion/ligation steps and extensive restriction mapping, which has imposed limitations...

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Detalles Bibliográficos
Publicado en:PLoS One
Autores principales: Yoshimatsu, Sho, Sone, Takefumi, Nakajima, Mayutaka, Sato, Tsukika, Okochi, Ryotaro, Ishikawa, Mitsuru, Nakamura, Mari, Sasaki, Erika, Shiozawa, Seiji, Okano, Hideyuki
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6711506/
https://ncbi.nlm.nih.gov/pubmed/31454364
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0221164
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