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An optimized protocol for isolation of high‐quality RNA through laser capture microdissection of leaf material

Laser Capture Microdissection is a powerful tool that allows thin slices of specific cell types to be separated from one another. However, the most commonly used protocol, which involves embedding tissue in paraffin wax, results in severely degraded RNA. Yields from low abundance cell types of leave...

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Vydáno v:Plant Direct
Hlavní autoři: Hua, Lei, Hibberd, Julian M.
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6710646/
https://ncbi.nlm.nih.gov/pubmed/31468025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pld3.156
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