An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

BACKGROUND: Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. The chal...

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Bibliographic Details
Published in:Plant Methods
Main Authors: Anjam, Muhammad Shahzad, Ludwig, Yvonne, Hochholdinger, Frank, Miyaura, Chisato, Inada, Masaki, Siddique, Shahid, Grundler, Florian M. W.
Format: Artigo
Language:Inglês
Published: BioMed Central 2016
Subjects:
Methodology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4847226/
https://ncbi.nlm.nih.gov/pubmed/27123040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-016-0123-9
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