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Heterologous Expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding
High level expression of many eukaryotic proteins for structural analysis is likely to require a eukaryotic host since many proteins are either insoluble or lack essential post-translational modifications when expressed in E. coli. The well-studied eukaryote Saccharomyces cerevisiae possesses severa...
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| Publié dans: | J Struct Funct Genomics |
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| Auteurs principaux: | , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6703162/ https://ncbi.nlm.nih.gov/pubmed/19701618 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-009-9068-9 |
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