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Optimization of cerebellar Purkinje neuron cultures and development of a plasmid-based method for Purkinje neuron-specific, miRNA-mediated protein knockdown
We present a simple and efficient method to knockdown proteins specifically in Purkinje Neurons (PN) present in mixed mouse primary cerebellar cultures. This method utilizes the introduction via nucleofection of a plasmid encoding a specific miRNA downstream of the L7/Pcp2 promoter, which drives PN-...
Gardado en:
| Publicado en: | Methods Cell Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6699752/ https://ncbi.nlm.nih.gov/pubmed/26794514 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.06.004 |
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