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Structural intermediates of a DNA–ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation

DNA ligases join adjacent 5′ phosphate (5′P) and 3′ hydroxyl (3′OH) termini of double-stranded DNA via a three-step mechanism requiring a nucleotide cofactor and divalent metal ion. Although considerable structural detail is available for the first two steps, less is known about step 3 where the DNA...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Williamson, Adele, Leiros, Hanna-Kirsti S
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698739/
https://ncbi.nlm.nih.gov/pubmed/31312841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz596
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