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Kinetics of T3-DNA Ligase-Catalyzed Phosphodiester Bond Formation Measured using the α-Hemolysin Nanopore

The latch region of the wild-type α-hemolysin (α-HL) protein channel can be used to distinguish single base modifications in double-stranded DNA (dsDNA) via ion channel measurements upon electrophoretic capture of dsDNA in the vestibule of α-HL. Herein, we investigated the use of the latch region to...

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Publicat a:ACS Nano
Autors principals: Tan, Cherie S., Riedl, Jan, Fleming, Aaron M., Burrows, Cynthia J., White, Henry S.
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5302010/
https://ncbi.nlm.nih.gov/pubmed/28024377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsnano.6b05995
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