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Triple Fluorescence Anisotropy Reporter Imaging in Living Cells
FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells. While emission ratios are generally useful for quantitative studies, they leave little room in the optical spectrum for additional...
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| Publié dans: | Bio Protoc |
|---|---|
| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Bio-Protocol
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6697128/ https://ncbi.nlm.nih.gov/pubmed/31423459 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3226 |
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