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Mapping of fluorescence anisotropy in living cells by ratio imaging. Application to cytoplasmic viscosity.
Steady-state and time-resolved fluorescence properties of probes incorporated into living cells give information about the microenvironment near the probe. We have extended studies of spatially averaged fluorescence anisotropy (r) by using an epifluorescence microscope, equipped with excitation and...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1990
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1280665/ https://ncbi.nlm.nih.gov/pubmed/2317548 |
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