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Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

A transcription factor (TF) is a protein that regulates gene expression by interacting with the RNA polymerase, another TF, and/or template DNA. GrgA is a novel transcription activator found specifically in the obligate intracellular bacterial pathogen Chlamydia. Protein pulldown assays using affini...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Desai, Malhar, Di, Rong, Fan, Huizhou
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6693641/
https://ncbi.nlm.nih.gov/pubmed/31403627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59687
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