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A stepwise mechanism for aqueous two-phase system formation in concentrated antibody solutions

Aqueous two-phase system (ATPS) formation is the macroscopic completion of liquid–liquid phase separation (LLPS), a process by which aqueous solutions demix into 2 distinct phases. We report the temperature-dependent kinetics of ATPS formation for solutions containing a monoclonal antibody and polye...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Rogers, Bradley A., Rembert, Kelvin B., Poyton, Matthew F., Okur, Halil I., Kale, Amanda R., Yang, Tinglu, Zhang, Jifeng, Cremer, Paul S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6690023/
https://ncbi.nlm.nih.gov/pubmed/31337677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1900886116
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