Scalable conjugation and characterization of immunoglobulins with stable mass isotope reporters for single-cell mass cytometry analysis

The advent of mass cytometry (CyTOF®) has permitted simultaneous detection of more than 40 antibody parameters at the single-cell level, although a limited number of metal-labeled antibodies are commercially available. Here we present optimized and scalable protocols for conjugation of lanthanide as...

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Veröffentlicht in:Methods Mol Biol
Hauptverfasser: Hartmann, Felix J., Simonds, Erin F., Vivanco, Nora, Bruce, Trevor, Borges, Luciene, Nolan, Garry P., Spitzer, Matthew H., Bendall, Sean C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6687300/
https://ncbi.nlm.nih.gov/pubmed/31077099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-9454-0_5
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