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Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization
The isolation of high‐quality RNA from the intervertebral disc and especially from the nucleus pulposus is challenging due to the low cellularity and high proteoglycan content of this tissue. In this study, we report a simple modification of the standard guanidinium thiocyanate‐phenol‐chloroform ext...
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| Pubblicato in: | JOR Spine |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
John Wiley & Sons, Inc.
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6686795/ https://ncbi.nlm.nih.gov/pubmed/31463444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jsp2.1017 |
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