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Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation.

Caged compounds enable fast, light-induced, and spatially-defined application of bioactive molecules to cells. Covalent attachment of a caging chromophore to a crucial functionality of a biomolecule renders it inert, while short pulses of light release the caged molecule in its active form. Caged ne...

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Publicado en:Methods Enzymol
Autores principales: Passlick, Stefan, Ellis-Davies, Graham C.R.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6684293/
https://ncbi.nlm.nih.gov/pubmed/31370929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.05.003
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