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Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
[Image: see text] Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G(4)Y), mono-functionalization of the monoclonal antibody trastuz...
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| Publicado no: | ACS Omega |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical Society
2019
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6682001/ https://ncbi.nlm.nih.gov/pubmed/31460288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.9b01727 |
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