Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

Proteins that contain disulfide bonds mainly mature in the oxidative environment of the eukaryotic endoplasmic reticulum or the periplasm of Gram-negative bacteria. In E. coli, disulfide bond containing recombinant proteins are often targeted to the periplasm by an N-terminal signal peptide that is...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Karyolaimos, Alexandros, Ampah-Korsah, Henry, Hillenaar, Tamara, Mestre Borras, Anna, Dolata, Katarzyna Magdalena, Sievers, Susanne, Riedel, Katharina, Daniels, Robert, de Gier, Jan-Willem
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6664373/
https://ncbi.nlm.nih.gov/pubmed/31396164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.01511
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